S3I-M2001

Name Value
Weight 508.511 g/mol
Zinc ID ZINC14973109
Smiles CCCCCCNC(=O)c1nc(-c2cccc3ccccc23)oc1Cc1ccc(OP(=O)(O)O)cc1
Molecular formula C27H29N2O6P
Mode of inhibition Direct STAT SH2; Non-peptide small molecule
CAS PC-62347

Clinical Trials

Study title Status Conditions Link

Visualization

References

Publication DOI Pubmed PMCID
An Oxazole-Based Small-Molecule Stat3 Inhibitor Modulates Stat3 Stability and Processing and Induces Antitumor Cell Effects 10.1021/cb7001973 N/A N/A

Vendors

Company Lnk to product
ProbeChem http://www.probechem.com/products_S3I-M2001.aspx

Filter data

Compound S3I-M2001
Experiment types Colony formation assay , EMSA, Flow cytometry , Immunofluorescce, Luciferase reporter measurements, Migration assay , TUNEL, Tumor volume, WST-1, Western Blot
Concentrations 0.30, 1.00, 10.00, 100.00, 150.00, 20.00, 200.00, 250.00, 30.00, 300.00, 350.00, 5.00, 50.00, 500.00, 800.00
Cell lines 3T3/v-Src, 3T3/v-Src/ ST3-SH2, 3T3/v-Src/ pcDNA3, HUVEC, MCF-10A, MDA-MB-231, MDA-MB-231 (xenograft), MDA-MB-435, MDA-MB-453, MiaPaca-1, NIH 3T3, NIH 3T3/hEGFR, NIH 3T3/v-Src, NIH3T3, NIH3T3/hEGFR, NIH3T3/v- Src, NIH3T3/v-Ras, NIH3T3/v-Src, NIH3T3/v-Src/GFP, NIH3T3/v-Src/Stat3-YFP, Panc-1, Sf-9
Organisms Homo sapiens, Mus musculus, Spodoptera frugiperda
Animal models athymic nude mice
STAT proteins STAT1:STAT1, STAT1:STAT3, STAT3, STAT3:STAT1, STAT3:STAT3, p-STAT3, p-STAT3(Y705)
Other Bcl-xL, Erk1/2, JAK1, Scr, YFP, p-ERK1/2, p-JAK1, p-Src, β-actin

Experiments

Compound Experiment type Investigated event Concentration Cell line Organism Animal model Stat protein Details
S3I-M2001 EMSA Influence on protein binding to DNA 0.30 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 1.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 10.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 30.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 300.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 500.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 0.30 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 1.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 10.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 30.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 300.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 500.00 µM Sf-9 Spodoptera frugiperda STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 1.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 10.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 30.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 300.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 500.00 µM Sf-9 Spodoptera frugiperda STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 10.00 µM NIH 3T3/v-Src Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 30.00 µM NIH 3T3/v-Src Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM NIH 3T3/v-Src Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 300.00 µM NIH 3T3/v-Src Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 10.00 µM MDA-MB-231 Homo sapiens STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 30.00 µM MDA-MB-231 Homo sapiens STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM MDA-MB-231 Homo sapiens STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 300.00 µM MDA-MB-231 Homo sapiens STAT3:STAT3
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM NIH 3T3/hEGFR Mus musculus STAT3:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM NIH 3T3/hEGFR Mus musculus STAT1:STAT1
S3I-M2001 EMSA Influence on protein binding to DNA 100.00 µM NIH 3T3/hEGFR Mus musculus STAT3:STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus p-STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM MDA-MB-435 Homo sapiens p-STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM MDA-MB-435 Homo sapiens STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM MDA-MB-435 Homo sapiens
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH 3T3/v-Src Mus musculus
S3I-M2001 Luciferase reporter measurements Influence on protein activity 30.00 µM NIH 3T3 Mus musculus
S3I-M2001 EMSA Influence on protein activity 30.00 µM NIH 3T3 Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein activity 100.00 µM NIH 3T3 Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein activity 300.00 µM NIH 3T3 Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein activity 800.00 µM NIH 3T3 Mus musculus STAT3:STAT3
S3I-M2001 EMSA Influence on protein activity 30.00 µM NIH 3T3 Mus musculus STAT1:STAT3
S3I-M2001 EMSA Influence on protein activity 100.00 µM NIH 3T3 Mus musculus STAT1:STAT3
S3I-M2001 EMSA Influence on protein activity 300.00 µM NIH 3T3 Mus musculus STAT1:STAT3
S3I-M2001 EMSA Influence on protein activity 800.00 µM NIH 3T3 Mus musculus STAT1:STAT3
S3I-M2001 EMSA Influence on protein activity 30.00 µM NIH 3T3 Mus musculus STAT1:STAT1
S3I-M2001 EMSA Influence on protein activity 100.00 µM NIH 3T3 Mus musculus STAT1:STAT1
S3I-M2001 EMSA Influence on protein activity 300.00 µM NIH 3T3 Mus musculus STAT1:STAT1
S3I-M2001 EMSA Influence on protein activity 800.00 µM NIH 3T3 Mus musculus STAT1:STAT1
S3I-M2001 Immunofluorescce Influence on protein translocation 100.00 µM NIH3T3/hEGFR Mus musculus STAT3
S3I-M2001 Immunofluorescce Influence on protein translocation 300.00 µM NIH3T3/v-Src/Stat3-YFP Mus musculus STAT3
S3I-M2001 Immunofluorescce Influence on protein translocation 300.00 µM NIH3T3/v-Src/GFP Mus musculus STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH3T3/v-Src/Stat3-YFP Mus musculus
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH3T3/v-Src/Stat3-YFP Mus musculus STAT3
S3I-M2001 Western Blot Influence on protein activity 100.00 µM NIH3T3/v-Src/Stat3-YFP Mus musculus
S3I-M2001 Immunofluorescce Influence on protein aggregation into perinuclear aggresomes 100.00 µM NIH3T3/v- Src Mus musculus STAT3
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM NIH3T3 Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM Panc-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 50.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 100.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 150.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 200.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 250.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 300.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 WST-1 Influence on cell proliferation 350.00 µM MiaPaca-1 Homo sapiens
S3I-M2001 Colony formation assay Influence on cell proliferation 30.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 Colony formation assay Influence on cell proliferation 100.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 Colony formation assay Influence on cell proliferation 30.00 µM NIH3T3/v-Ras Mus musculus
S3I-M2001 Colony formation assay Influence on cell proliferation 100.00 µM NIH3T3/v-Ras Mus musculus
S3I-M2001 Colony formation assay Influence on cell proliferation 30.00 µM Panc-1 Homo sapiens
S3I-M2001 Colony formation assay Influence on cell proliferation 100.00 µM Panc-1 Homo sapiens
S3I-M2001 TUNEL Influence on DNA 50.00 µM NIH3T3 Mus musculus
S3I-M2001 TUNEL Influence on DNA 50.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 TUNEL Influence on DNA 50.00 µM HUVEC Homo sapiens
S3I-M2001 TUNEL Influence on DNA 50.00 µM MDA-MB-453 Homo sapiens
S3I-M2001 TUNEL Influence on DNA 50.00 µM MCF-10A Mus musculus
S3I-M2001 TUNEL Influence on DNA 50.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 TUNEL Influence on DNA 50.00 µM MDA-MB-435 Homo sapiens
S3I-M2001 Flow cytometry Influence on apoptosis 50.00 µM 3T3/v-Src/ pcDNA3 Mus musculus
S3I-M2001 Flow cytometry Influence on apoptosis 50.00 µM 3T3/v-Src/ ST3-SH2 Mus musculus
S3I-M2001 Flow cytometry Influence on apoptosis 50.00 µM 3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on apoptosis 50.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on apoptosis 50.00 µM MDA-MB-435 Homo sapiens
S3I-M2001 Western Blot Influence on apoptosis 50.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 Western Blot Influence on apoptosis 50.00 µM MDA-MB-435 Homo sapiens
S3I-M2001 Migration assay Influence on cell motility 100.00 µM NIH3T3/v-Src Mus musculus
S3I-M2001 Migration assay Influence on cell motility 100.00 µM MDA-MB-231 Homo sapiens
S3I-M2001 Migration assay Influence on cell motility 100.00 µM Panc-1 Homo sapiens
S3I-M2001 Tumor volume Influence on tumor growth 5.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice
S3I-M2001 Tumor volume Influence on tumor growth 10.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice
S3I-M2001 Tumor volume Influence on tumor growth 20.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice
S3I-M2001 EMSA Influence on DNA binding in tumor 5.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice STAT3:STAT3
S3I-M2001 Western Blot Influence on protein activity in tumor tissue 5.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice STAT3
S3I-M2001 Western Blot Influence on protein activity in tumor tissue 5.00 mg/kg MDA-MB-231 (xenograft) Mus musculus athymic nude mice p-STAT3(Y705)