Auranofin

Name Value
Weight 678.483 g/mol
Zinc ID N/A
Smiles CCP(=[Au]S[C@H]1[C@@H]([C@H]([C@@H]([C@H](O1)COC(=O)C)OC(=O)C)OC(=O)C)OC(=O)C)(CC)CC
Molecular formula C20H34AuO9PS
Mode of inhibition Direct JAK1; indirect STAT3; Tyrosine kinase inhibitor
CAS 34031-32-8

Clinical Trials

Study title Status Conditions Link
Oral Auranofin for Reduction of Latent Viral Reservoir in Patients With HIV Infection Withdrawn HIV NCT02176135
Auranofin PK Following Oral Dose Administration Completed Amoebiasis NCT02089048
Auranofin in Treating Patients With Recurrent Epithelial Ovarian, Primary Peritoneal, or Fallopian Tube Cancer Completed Recurrent Fallopian Tube Cancer|Recurrent Ovarian Epithelial Cancer|Recurrent Primary Peritoneal Cavity Cancer NCT01747798
Phase I and II Study of Auranofin in Chronic Lymphocytic Leukemia (CLL) Completed Chronic Lymphocytic Leukemia (CLL)|Small Lymphocytic Lymphoma|Leukemia, Prolymphocytic NCT01419691
Auranofin and Sirolimus in Treating Participants With Ovarian Cancer Active, not recruiting Ovarian Serous Tumor|Recurrent Ovarian Carcinoma NCT03456700
Auranofin for Giardia Protozoa Recruiting Amoebic Dysentery|Giardiasis NCT02736968
Auranofin in Decreasing Pain in Patients With Paclitaxel-Induced Pain Syndrome Completed Pain NCT02063698
Auranofin and Sirolimus in Treating Patients With Advanced Solid Tumors or Recurrent Non-Small Cell Lung Cancer Withdrawn Recurrent Non-small Cell Lung Cancer|Unspecified Adult Solid Tumor, Protocol Specific NCT02126527
Sirolimus and Auranofin in Treating Patients With Advanced or Recurrent Non-Small Cell Lung Cancer or Small Cell Lung Cancer Recruiting Extensive Stage Small Cell Lung Carcinoma|Lung Adenocarcinoma|Recurrent Non-Small Cell Lung Carcinoma|Recurrent Small Cell Lung Carcinoma|Squamous Cell Lung Carcinoma|Stage IIIA Non-Small Cell Lung Cancer|Stage IIIB Non-Small Cell Lung Cancer|Stage IV Non-Small Cell Lung Cancer NCT01737502
Multi Interventional Study Exploring HIV-1 Residual Replication: a Step Towards HIV-1 Eradication and Sterilizing Cure Active, not recruiting Chronic Infection|HIV NCT02961829
TB Host Directed Therapy Active, not recruiting Tuberculosis NCT02968927
A Proof-of-concept Clinical Trial Assessing the Safety of the Coordinated Undermining of Survival Paths by 9 Repurposed Drugs Combined With Metronomic Temozolomide (CUSP9v3 Treatment Protocol) for Recurrent Glioblastoma Active, not recruiting Glioblastoma NCT02770378
Comparative Analysis of Outcomes Among Patients Initiating Xeljanz in Combination With Oral MTX Who Withdraw MTX Versus Continue MTX Completed Rheumatoid Arthritis NCT03975790
An Observational Study of MabThera/Rituxan (Rituximab) and Alternative TNF-Inhibitors in Patients With Rheumatoid Arthritis and an Inadequate Response to a Single Previous TNF-Inhibitor Completed Rheumatoid Arthritis NCT01557348

References

Publication DOI Pubmed PMCID
Auranofin blocks interleukin-6 signalling by inhibiting phosphorylation of JAK1 and STAT3. Immunology Nam-Hoon Kim, Mun-Yong Lee, Seon-Joo Park, Jeong-Sun Choi, Mi-Kyung Oh, In-Sook Kim, 122, 4, None, 2007-07-20, 10.1111/j.1365-2567.2007.02679.x 10.1111/j.1365-2567.2007.02679.x 17645497 PMC2266044
The gold compound auranofin induces apoptosis of human multiple myeloma cells through both down-regulation of STAT3 and inhibition of NF-κB activity. Leukemia research Aya Nakaya, Morihiko Sagawa, Akihiro Muto, Hideo Uchida, Yasuo Ikeda, Masahiro Kizaki, 35, 2, None, 2010-06-12, 10.1016/j.leukres.2010.05.011 10.1016/j.leukres.2010.05.011 20542334 20542334
The cartilaginous skeleton of the bronchial tree. Advances in anatomy, embryology, and cell biology F Vanpeperstraete, 48, 3, None, None, 10.5483/BMBRep.2013.46.1.123 10.5483/BMBRep.2013.46.1.123 4133824 PMC4133824
Anti-rheumatic agent auranofin induced apoptosis in chronic myeloid leukemia cells resistant to imatinib through both Bcr/Abl-dependent and -independent mechanisms N/A N/A N/A

Vendors

Company Lnk to product
Tocris https://www.tocris.com/products/auranofin_4600
MERCK (Sigma Aldrich) https://www.sigmaaldrich.com/catalog/product/sigma/a6733?lang=pl&region=PL
AK Scientific Inc. https://aksci.com/item_detail.php?cat=V0192
Caymanchemical https://www.caymanchem.com/product/15316/auranofin

Filter data

Compound Auranofin
Experiment types Colony Formation , ELISA, Flow cytometry Annexin V-FITC, Flow cytometry DNA content, Giemsa staining, Immunocytochemistry, Immunofluorescence, MTS, MTS Assay , MTT Assay , RT-qPCR, TRAPeze telomerase detection, Western Blot
Concentrations 0.05, 0.10, 0.30, 0.50, 1.00, 2.00
Cell lines IM9, KBM5, KBM5-T315I, MDA-MB 231, RPM8226, U266, U266 pEGFP-C1-mcl-1, U266 pEGFP-C1-neo
Organisms Homo sapiens
Animal models
STAT proteins STAT3, STAT5, p-STAT3
Other Akt, Annexin, Bax, Bcl-2, Bcl-xL, Bcr-Abl, Cleaved caspase 3, Cleaved caspase 8, Cleaved caspase 9, Erk1/2, IKKα, IκBα, JAK2, Mcl-1, NF-κB, Pro-caspase 3, Pro-caspase 8, Pro-caspase 9, Rb, Survivn , XIAP, gp130, p-IκBα, p-JAK2, p-Rb, p-gp130, p21, telomerase products, β-actin

Experiments

Compound Experiment type Investigated event Concentration Cell line Organism Animal model Stat protein Details
Auranofin MTS Influence on cell viability 0.05 µM U266 Homo sapiens
Auranofin MTS Influence on cell viability 0.10 µM U266 Homo sapiens
Auranofin MTS Influence on cell viability 0.50 µM U266 Homo sapiens
Auranofin MTS Influence on cell viability 1.00 µM U266 Homo sapiens
Auranofin MTS Influence on cell viability 0.05 µM RPM8226 Homo sapiens
Auranofin MTS Influence on cell viability 0.10 µM RPM8226 Homo sapiens
Auranofin MTS Influence on cell viability 0.50 µM RPM8226 Homo sapiens
Auranofin MTS Influence on cell viability 1.00 µM RPM8226 Homo sapiens
Auranofin MTS Influence on cell viability 0.05 µM IM9 Homo sapiens
Auranofin MTS Influence on cell viability 0.10 µM IM9 Homo sapiens
Auranofin MTS Influence on cell viability 0.50 µM IM9 Homo sapiens
Auranofin MTS Influence on cell viability 1.00 µM IM9 Homo sapiens
Auranofin Giemsa staining Influence on cell morphology 0.05 µM U266 Homo sapiens
Auranofin Flow cytometry Annexin V-FITC Influence on apoptosis 0.05 µM U266 Homo sapiens
Auranofin Flow cytometry DNA content Influence on cell cycle 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on cell cycle 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on cell cycle 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on cell cycle 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on cell cycle 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on caspase activation 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on apoptosis-associated proteins in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on apoptosis-associated proteins in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on apoptosis-associated proteins in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on apoptosis-associated proteins in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on apoptosis-associated proteins in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.05 µM U266 Homo sapiens p-STAT3
Auranofin Western Blot Influence on protein activity 0.10 µM U266 Homo sapiens p-STAT3
Auranofin Western Blot Influence on protein activity 0.05 µM U266 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity 0.10 µM U266 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity in presence of IL-6 0.05 µM U266 Homo sapiens p-STAT3
Auranofin Immunofluorescence Influence on apoptosis in transfected cells 0.05 µM U266 pEGFP-C1-neo Homo sapiens
Auranofin Immunofluorescence Influence on apoptosis in transfected cells 0.05 µM U266 pEGFP-C1-mcl-1 Homo sapiens
Auranofin MTS Influence on cell viability 0.05 µM U266 pEGFP-C1-neo Homo sapiens
Auranofin MTS Influence on cell viability 0.05 µM U266 pEGFP-C1-mcl-1 Homo sapiens
Auranofin ELISA Influence on protein-DNA interaction 0.05 µM U266 Homo sapiens
Auranofin ELISA Influence on protein-DNA interaction 0.10 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of TNF-α 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of TNF-α 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of TNF-α 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of TNF-α 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of SN50 0.05 µM U266 Homo sapiens
Auranofin Western Blot Influence on protein activity in presence of SN50 0.05 µM U266 Homo sapiens
Auranofin MTT Assay Influence on cell proliferation 0.30 µM MDA-MB 231 Homo sapiens
Auranofin MTT Assay Influence on cell proliferation 0.50 µM MDA-MB 231 Homo sapiens
Auranofin MTT Assay Influence on cell proliferation 1.00 µM MDA-MB 231 Homo sapiens
Auranofin MTT Assay Influence on cell proliferation 2.00 µM MDA-MB 231 Homo sapiens
Auranofin MTT Assay Influence on cell proliferation in presence of NAC 1.00 µM MDA-MB 231 Homo sapiens
Auranofin Immunocytochemistry Influence on cell proliferation 0.50 µM MDA-MB 231 Homo sapiens
Auranofin Immunocytochemistry Influence on cell proliferation 1.00 µM MDA-MB 231 Homo sapiens
Auranofin Colony Formation Influence on cell growth 0.50 µM MDA-MB 231 Homo sapiens
Auranofin Colony Formation Influence on cell growth 1.00 µM MDA-MB 231 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.30 µM MDA-MB 231 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity 0.50 µM MDA-MB 231 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity 1.00 µM MDA-MB 231 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity 0.30 µM MDA-MB 231 Homo sapiens p-STAT3
Auranofin Western Blot Influence on protein activity 0.50 µM MDA-MB 231 Homo sapiens p-STAT3
Auranofin Western Blot Influence on protein activity 1.00 µM MDA-MB 231 Homo sapiens p-STAT3
Auranofin Western Blot Influence on protein activity in presence of NAC 1.00 µM MDA-MB 231 Homo sapiens STAT3
Auranofin Western Blot Influence on protein activity in presence of NAC 1.00 µM MDA-MB 231 Homo sapiens p-STAT3
Auranofin TRAPeze telomerase detection Influence on protein activity in presence of NAC 1.00 µM MDA-MB 231 Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 0.50 µM KBM5 Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 1.00 µM KBM5 Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 2.00 µM KBM5 Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 0.50 µM KBM5-T315I Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 1.00 µM KBM5-T315I Homo sapiens
Auranofin MTS Assay Influence on cell proliferation 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens STAT5
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5 Homo sapiens
Auranofin Western Blot Influence on protein activity 0.50 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 1.00 µM KBM5-T315I Homo sapiens
Auranofin Western Blot Influence on protein activity 2.00 µM KBM5-T315I Homo sapiens
Auranofin RT-qPCR 0.50 µM KBM5 Homo sapiens
Auranofin RT-qPCR 1.00 µM KBM5 Homo sapiens
Auranofin RT-qPCR 2.00 µM KBM5 Homo sapiens
Auranofin RT-qPCR 0.50 µM KBM5-T315I Homo sapiens
Auranofin RT-qPCR 1.00 µM KBM5-T315I Homo sapiens
Auranofin RT-qPCR 2.00 µM KBM5-T315I Homo sapiens