inS3-54A18

Name Value
Weight 373.839 g/mol
Zinc ID ZINC2301101
Smiles O=C1/C(=C\c2ccc(Cl)cc2)C=C(c2ccccc2)N1c1ccc(O)cc1
Molecular formula C23H16ClNO2
Mode of inhibition Direct STAT DBD;
CAS 328998-53-4

Clinical Trials

Study title Status Conditions Link

Visualization

References

Publication DOI Pubmed PMCID
Small-molecule inhibitors targeting the DNA-binding domain of STAT3 suppress tumor growth, metastasis and STAT3 target gene expression in vivo. Oncogene W Huang, Z Dong, Y Chen, F Wang, C J Wang, H Peng, Y He, G Hangoc, K Pollok, G Sandusky, X-Y Fu, H E Broxmeyer, Z-Y Zhang, J-Y Liu, J-T Zhang, 35, 6, 2016-02-11, 2015-06-15, 10.1038/onc.2015.215 10.1038/onc.2015.215 26073084 26073084

Vendors

Company Lnk to product
MedChemExpress https://www.medchemexpress.com/inS3-54A18.html
ProbeChem http://www.probechem.com/products_InS3-54A18.aspx
ChemScene https://www.chemscene.com/328998-53-4.html

Filter data

Compound inS3-54A18
Experiment types Body weight, Chromatin immunoprecipitation assay, Colony formation assay , ELISA, EMSA, Immunohistochemistry, Invasion assay , Organ weight, Sulforhodamine B assay, Tumor volume, Tumor weight, Western Blot, Wound healing assay
Concentrations 10.00, 100.00, 13.00, 20.00, 200.00, 25.00, 5.00, 50.00, N/A
Cell lines A549, A549 (xenograft), BFU-E, CFU-GEMM, CFU-GM , H1299, IMR90, MCF10A1, MDA-MB-231, MDA-MB-438
Organisms Homo sapiens, Mus musculus
Animal models NOD/SCID
STAT proteins STAT1, STAT3, STAT3 (chromatin bound), STAT3 (cytosol), STAT3 (soluble nuclear), p-STAT3
Other Actin, Actin (chromatin bound), Actin (cytosol), Actin (soluble nuclear), Histone (chromatin bound), Histone (cytosol), Histone (soluble nuclear), Survivin, Tubulin (chromatin bound), Tubulin (cytosol), Tubulin (soluble nuclear), VEGF

Experiments

Compound Experiment type Investigated event Concentration Cell line Organism Animal model Stat protein Details
inS3-54A18 Colony formation assay Influence on cell motility 20.00 µM CFU-GEMM Homo sapiens
inS3-54A18 Colony formation assay Influence on cell motility 20.00 µM CFU-GM Homo sapiens
inS3-54A18 Colony formation assay Influence on cell motility 20.00 µM BFU-E Homo sapiens
inS3-54A18 EMSA Influence on protein binding to DNA 5.00 µM H1299 Homo sapiens STAT1
inS3-54A18 EMSA Influence on protein binding to DNA 13.00 µM H1299 Homo sapiens STAT1
inS3-54A18 EMSA Influence on protein binding to DNA 25.00 µM H1299 Homo sapiens STAT1
inS3-54A18 EMSA Influence on protein binding to DNA 50.00 µM H1299 Homo sapiens STAT1
inS3-54A18 EMSA Influence on protein binding to DNA 100.00 µM H1299 Homo sapiens STAT1
inS3-54A18 EMSA Influence on protein binding to DNA 5.00 µM H1299 Homo sapiens STAT3
inS3-54A18 EMSA Influence on protein binding to DNA 13.00 µM H1299 Homo sapiens STAT3
inS3-54A18 EMSA Influence on protein binding to DNA 25.00 µM H1299 Homo sapiens STAT3
inS3-54A18 EMSA Influence on protein binding to DNA 50.00 µM H1299 Homo sapiens STAT3
inS3-54A18 EMSA Influence on protein binding to DNA 100.00 µM H1299 Homo sapiens STAT3
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A IMR90 Homo sapiens
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A A549 Homo sapiens
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A H1299 Homo sapiens
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A MCF10A1 Homo sapiens
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A MDA-MB-231 Homo sapiens
inS3-54A18 Sulforhodamine B assay Influence on cell viability N/A N/A MDA-MB-438 Homo sapiens
inS3-54A18 ELISA Influence on cell viability 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 ELISA Influence on cell viability 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 ELISA Influence on cell viability 20.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Wound healing assay Influence on cell motility 5.00 µM A549 Homo sapiens
inS3-54A18 Wound healing assay Influence on cell motility 10.00 µM A549 Homo sapiens
inS3-54A18 Wound healing assay Influence on cell motility 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Wound healing assay Influence on cell motility 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Invasion assay Influence on cell motility 5.00 µM A549 Homo sapiens
inS3-54A18 Invasion assay Influence on cell motility 10.00 µM A549 Homo sapiens
inS3-54A18 Invasion assay Influence on cell motility 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Invasion assay Influence on cell motility 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens STAT3 (cytosol)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens STAT3 (cytosol)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens STAT3 (cytosol)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens STAT3 (cytosol)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens STAT3 (soluble nuclear)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens STAT3 (soluble nuclear)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens STAT3 (soluble nuclear)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens STAT3 (soluble nuclear)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens STAT3 (chromatin bound)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens STAT3 (chromatin bound)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens STAT3 (chromatin bound)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens STAT3 (chromatin bound)
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM A549 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 5.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Chromatin immunoprecipitation assay Influence on protein activity 10.00 µM MDA-MB-231 Homo sapiens
inS3-54A18 Western Blot Influence on protein activity in presence of IL-6 10.00 µM A549 Homo sapiens p-STAT3
inS3-54A18 Western Blot Influence on protein activity in presence of IL-6 10.00 µM A549 Homo sapiens STAT3
inS3-54A18 Western Blot Influence on protein activity in presence of IL-6 10.00 µM A549 Homo sapiens
inS3-54A18 Western Blot Influence on protein activity in presence of IL-6 10.00 µM A549 Homo sapiens
inS3-54A18 Tumor volume Influence on tumor growth 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID
inS3-54A18 Body weight Influence on tumor growth 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID
inS3-54A18 Tumor weight Influence on tumor growth 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID
inS3-54A18 Organ weight Influence on tumor growth 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID
inS3-54A18 Immunohistochemistry Influence on protein activity 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID STAT3
inS3-54A18 Immunohistochemistry Influence on protein activity 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID
inS3-54A18 Immunohistochemistry Influence on protein activity 200.00 mg/kg A549 (xenograft) Mus musculus NOD/SCID