Celastrol

Name Value
Weight 450.619 g/mol
Zinc ID ZINC19795938
Smiles CC1=C(O)C(=O)C=C2C1=CC=C1[C@@]3(C)CC[C@@]4(C)CC[C@@](C)(C(=O)O)C[C@H]4[C@]3(C)CC[C@@]21C
Molecular formula C29H38O4
Mode of inhibition Direct JAK2, indirect SRC and STAT; Natural product
CAS 34157-83-0

Clinical Trials

Study title Status Conditions Link

References

Publication DOI Pubmed PMCID
Celastrol inhibits dengue virus replication via up-regulating type I interferon and downstream interferon-stimulated responses. Antiviral research Jung-Sheng Yu, Chin-Kai Tseng, Chun-Kuang Lin, Yao-Chin Hsu, Yu-Hsuan Wu, Ching-Liang Hsieh, Jin-Ching Lee, 137, None, None, 2016-11-12, 10.1016/j.antiviral.2016.11.010 10.1016/j.antiviral.2016.11.010 27847245 27847245
[The problems of senile osteoporosis from internist and geriatric point of view. II]. Das Deutsche Gesundheitswesen U J Schmidt, G Brüschke, I Grossmann, J Haase, R Ihle, I Kalbe, R Mühlbach, P Reitzig, 25, 13, 1970-03-31, None, 10.3892/etm.2017.4568 10.3892/etm.2017.4568 5488742 28673005
Celastrol suppresses expression of adhesion molecules and chemokines by inhibiting JNK-STAT1/NF-κB activation in poly(I:C)-stimulated astrocytes. BMB reports Soo Yeon An, Gi Soo Youn, Hyejin Kim, Soo Young Choi, Jinseu Park, 50, 1, None, None, 10.5483/bmbrep.2017.50.1.114 10.5483/bmbrep.2017.50.1.114 28027722 PMC5319661

Vendors

Company Lnk to product
Key Organics https://www.keyorganics.net/celastrol-mfcd03424073-34157-83-0-c29h38o4.html
TargetMol https://www.targetmol.com/compound/Celastrol
MERCK (Sigma Aldrich) https://www.sigmaaldrich.com/catalog/product/sigma/c0869?lang=pl&region=PL
Caymanchemical https://www.caymanchem.com/product/70950
Ambinter http://www.ambinter.com/reference/Amb10843188
Mcule https://mcule.com/MCULE-6960695081/
Selleckchem https://www.selleckchem.com/products/Celastrol.html
Tocris https://www.tocris.com/products/celastrol_3203
Mcule https://mcule.com/MCULE-3609209994/
Molport https://www.molport.com/shop/molecule-link/MolPort-003-665-482
AK Scientific Inc. https://aksci.com/item_detail.php?cat=S492
MedChemExpress https://www.medchemexpress.com/Celastrol.html
Matrix Scientific http://www.matrixscientific.com/168000.html

Filter data

Compound Celastrol
Experiment types ELISA, EMSA, Invasion assay , Luciferase activity, MTT, MTT Assay , RT-qPCR, Western Blot, Wound healing assay
Concentrations 0.05, 0.10, 0.12, 0.15, 0.20, 0.25, 0.30, 0.40, 0.50, 1.00, 2.00
Cell lines CRT-MG, Huh7, OVCAR-3, SKOV-3
Organisms Homo sapiens, Mus musculus
Animal models ICR suckling mice
STAT proteins STAT1, STAT2, p-STAT1, p-STAT2
Other Actin, Actin (cytosol), Actin (nuclei), CCL2, CXCL10, CXCL8, DENV1, DENV2, DENV3, DENV4, ERK, GAPDH, ICAM-1, IFN-α, IFN-α-17, IFN-α-2, IFN-α-5, ISRE, IkBα, IκBα (cytosol), JNK, MMP-2, MMP-7, MMP-9, NF-κB, NS2B, OAS1, OAS2, OAS3, PARP (cytosol), PARP (nuclei), VCAM-1, p-ERK, p-JNK, p-p38, p-p65, p65 (nucei), pIκBα (cytosol), pKKα, pKKα/β, pKKβ, β-actin

Experiments

Compound Experiment type Investigated event Concentration Cell line Organism Animal model Stat protein Details
Celastrol RT-qPCR Influence on RNA replication 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.20 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.05 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.10 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.15 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.20 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.05 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.10 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.15 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on protein synthesis 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence on RNA replication 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol ELISA Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol ELISA Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol ELISA Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol ELISA Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol Western Blot Influence on Jak-STAT signalling 0.20 µM Huh7 Homo sapiens p-STAT1
Celastrol Western Blot Influence on Jak-STAT signalling 0.20 µM Huh7 Homo sapiens STAT1
Celastrol Western Blot Influence on Jak-STAT signalling 0.20 µM Huh7 Homo sapiens p-STAT2
Celastrol Western Blot Influence on Jak-STAT signalling 0.20 µM Huh7 Homo sapiens STAT2
Celastrol Western Blot Influence on Jak-STAT signalling 0.20 µM Huh7 Homo sapiens
Celastrol Luciferase activity Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol Luciferase activity Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol Luciferase activity Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol Luciferase activity Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.05 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.10 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.15 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral IFN gene expression 0.20 µM Huh7 Homo sapiens
Celastrol RT-qPCR Influence of antiviral responses 0.10 mg/kg Mus musculus ICR suckling mice
Celastrol RT-qPCR Influence of antiviral responses 0.10 mg/kg Mus musculus ICR suckling mice
Celastrol RT-qPCR Influence of antiviral responses 0.10 mg/kg Mus musculus ICR suckling mice
Celastrol RT-qPCR Influence of antiviral responses 0.10 mg/kg Mus musculus ICR suckling mice
Celastrol RT-qPCR Influence of antiviral responses 0.10 mg/kg Mus musculus ICR suckling mice
Celastrol MTT Influence on cell proliferation 0.12 µM SKOV-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 0.25 µM SKOV-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 0.50 µM SKOV-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 1.00 µM SKOV-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 2.00 µM SKOV-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 0.12 µM OVCAR-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 0.25 µM OVCAR-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 0.50 µM OVCAR-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 1.00 µM OVCAR-3 Homo sapiens
Celastrol MTT Influence on cell proliferation 2.00 µM OVCAR-3 Homo sapiens
Celastrol Wound healing assay Influence on cell migration 0.12 µM OVCAR-3 Homo sapiens
Celastrol Wound healing assay Influence on cell migration 0.25 µM OVCAR-3 Homo sapiens
Celastrol Wound healing assay Influence on cell migration 0.50 µM OVCAR-3 Homo sapiens
Celastrol Invasion assay Influence on cell migration 0.12 µM OVCAR-3 Homo sapiens
Celastrol Invasion assay Influence on cell migration 0.25 µM OVCAR-3 Homo sapiens
Celastrol Invasion assay Influence on cell migration 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on protein activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.12 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.25 µM OVCAR-3 Homo sapiens
Celastrol Western Blot Influence on MMP activity 0.50 µM OVCAR-3 Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.05 μg/ml CRT-MG Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.10 μg/ml CRT-MG Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.20 μg/ml CRT-MG Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.30 μg/ml CRT-MG Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.40 μg/ml CRT-MG Homo sapiens
Celastrol MTT Assay Influence on cell proliferation 0.50 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.05 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.10 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.30 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.05 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.10 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of adhesion molecules 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on expression of adhesion molecules 0.30 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol RT-qPCR Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.05 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.10 μg/ml CRT-MG Homo sapiens
Celastrol ELISA Influence on expression of chemokines 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens p-STAT1
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens p-STAT1
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens p-STAT1
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens STAT1
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens STAT1
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens STAT1
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.05 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.10 μg/ml CRT-MG Homo sapiens
Celastrol Western Blot Influence on protein activity 0.30 μg/ml CRT-MG Homo sapiens
Celastrol EMSA Influence on protein binding to DNA 0.05 μg/ml CRT-MG Homo sapiens
Celastrol EMSA Influence on protein binding to DNA 0.10 μg/ml CRT-MG Homo sapiens
Celastrol EMSA Influence on protein binding to DNA 0.30 μg/ml CRT-MG Homo sapiens