Withaferin A

Name Value
Weight 470.606 g/mol
Zinc ID ZINC64373300
Smiles CC1=C(CO)C(=O)O[C@H]([C@@H](C)[C@H]2CC[C@H]3[C@@H]4C[C@H]5O[C@]56[C@@H](O)C=CC(=O)[C@]6(C)[C@H]4CC[C@@]32C)C1
Molecular formula C28H38O6
Mode of inhibition JAK2 and STAT; Natural product
CAS 5119-48-2

Clinical Trials

Study title Status Conditions Link
Withania Somnifera: an Immunomodulator and Anti-inflammatory Agent for Schizophrenia Completed Schizophrenia|Schizoaffective Disorder NCT01793935

References

Publication DOI Pubmed PMCID
Cytochemistry of phosphatases of the sarcoplasmic reticulum. II. In situ localization of the Mg-dependent enzyme. The Journal of cell biology L W Tice, A G Engel, 31, 3, None, None, 10.4137/BCI.S18863 10.4137/BCI.S18863 4226392 PMC4226392
[Studies on the enterococci in some perishable lactic acid products]. Veterinarno-meditsinski nauki V Aleksieva, 10, 10, None, None, 10.15430/JCP.2015.20.3.185 10.15430/JCP.2015.20.3.185 4597807 PMC4597807

Vendors

Company Lnk to product
Tocris https://www.tocris.com/products/withaferin-a_2816
Adooq https://www.adooq.com/withaferin-a.html
Caymanchemical https://www.caymanchem.com/product/11352/withaferin-a
MERCK (Sigma Aldrich) https://www.merckmillipore.com/PL/pl/product/Withaferin-A-Withania-somnifera-CAS-5119-48-2-Calbiochem,EMD_BIO-681535?ReferrerURL=https%3A%2F%2Fwww.google.com%2F&bd=1
MedChemExpress https://www.medchemexpress.com/Withaferin_A.html
Santa Cruz Biotechnology https://www.scbt.com/p/withaferin-a-5119-48-2

Filter data

Compound Withaferin A
Experiment types Body weight, Immunofluorescence, MTT, Staining with TOTO-3 Iodide, Transcriptional activity assay, Transwell migration assay, Tumor volume, Tumor weight, Western Blot, Wound healing assay
Concentrations 0.00, 0.01, 0.06, 0.10, 0.12, 0.25, 0.50, 0.62, 1.00, 1.25, 10.00, 2.00, 2.50, 5.00, N/A
Cell lines Be(2)-c, HCT116, HCT116 (xenograft), IMR-32, LAN-5, MM1.RL, SH-SY5Y, SMS-KCNR, U266
Organisms Homo sapiens, Mus musculus
Animal models BALB/c
STAT proteins STAT3, p-STAT3 (S727), p-STAT3 (Y705)
Other Histone H4, α-tubulin

Experiments

Compound Experiment type Investigated event Concentration Cell line Organism Animal model Stat protein Details
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM Be(2)-c Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM Be(2)-c Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM Be(2)-c Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM Be(2)-c Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM Be(2)-c Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM SMS-KCNR Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM SMS-KCNR Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM SMS-KCNR Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM SMS-KCNR Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM SMS-KCNR Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM SH-SY5Y Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM SH-SY5Y Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM SH-SY5Y Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM SH-SY5Y Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM SH-SY5Y Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM IMR-32 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM IMR-32 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM IMR-32 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM IMR-32 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM IMR-32 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM LAN-5 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM LAN-5 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM LAN-5 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM LAN-5 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM LAN-5 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM MM1.RL Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM MM1.RL Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM MM1.RL Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM MM1.RL Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM MM1.RL Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 0.62 µM U266 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 1.25 µM U266 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 2.50 µM U266 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 5.00 µM U266 Homo sapiens
Withaferin A Staining with TOTO-3 Iodide Influence on cell viability 10.00 µM U266 Homo sapiens
Withaferin A Western Blot Influence on protein activity 5.00 µM Be(2)-c Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity 5.00 µM Be(2)-c Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity 5.00 µM Be(2)-c Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity 5.00 µM Be(2)-c Homo sapiens
Withaferin A Western Blot Influence on protein activity 5.00 µM SMS-KCNR Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity 5.00 µM SMS-KCNR Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity 5.00 µM SMS-KCNR Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity 5.00 µM SMS-KCNR Homo sapiens
Withaferin A Western Blot Influence on protein activity 5.00 µM SH-SY5Y Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity 5.00 µM SH-SY5Y Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity 5.00 µM SH-SY5Y Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity 5.00 µM SH-SY5Y Homo sapiens
Withaferin A Western Blot Influence on protein activity 5.00 µM IMR-32 Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity 5.00 µM IMR-32 Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity 5.00 µM IMR-32 Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity 5.00 µM IMR-32 Homo sapiens
Withaferin A Western Blot Influence on protein activity 5.00 µM LAN-5 Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity 5.00 µM LAN-5 Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity 5.00 µM LAN-5 Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity 5.00 µM LAN-5 Homo sapiens
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM MM1.RL Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM MM1.RL Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM MM1.RL Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM MM1.RL Homo sapiens
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM U266 Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM U266 Homo sapiens p-STAT3 (S727)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM U266 Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity in presence of IL-6 10.00 µM U266 Homo sapiens
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM MM1.RL Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM MM1.RL Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM MM1.RL Homo sapiens
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM U266 Homo sapiens p-STAT3 (Y705)
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM U266 Homo sapiens STAT3
Withaferin A Western Blot Influence on protein activity in presence of IL-6 5.00 µM U266 Homo sapiens
Withaferin A MTT Influence on cell viability N/A µM HCT116 Homo sapiens
Withaferin A MTT Influence on cell proliferation 1.00 µM HCT116 Homo sapiens
Withaferin A Wound healing assay Influence on cells motility 0.00 µM HCT116 Homo sapiens
Withaferin A Wound healing assay Influence on cells motility 0.01 µM HCT116 Homo sapiens
Withaferin A Wound healing assay Influence on cells motility 0.10 µM HCT116 Homo sapiens
Withaferin A Wound healing assay Influence on cells motility 1.00 µM HCT116 Homo sapiens
Withaferin A Transwell migration assay Influence on cells motility 1.00 µM HCT116 Homo sapiens
Withaferin A Transcriptional activity assay Influence on protein transcriptional activity in presence of IL-6 0.06 µM HCT116 Homo sapiens
Withaferin A Transcriptional activity assay Influence on protein transcriptional activity in presence of IL-6 0.12 µM HCT116 Homo sapiens
Withaferin A Transcriptional activity assay Influence on protein transcriptional activity in presence of IL-6 0.25 µM HCT116 Homo sapiens
Withaferin A Transcriptional activity assay Influence on protein transcriptional activity in presence of IL-6 0.50 µM HCT116 Homo sapiens
Withaferin A Transcriptional activity assay Influence on protein transcriptional activity in presence of IL-6 1.00 µM HCT116 Homo sapiens
Withaferin A Immunofluorescence Influence on protein transcriptional activity in presence of IL-6 1.00 µM HCT116 Homo sapiens STAT3
Withaferin A Immunofluorescence Influence on protein transcriptional activity in presence of IL-6 1.00 µM HCT116 Homo sapiens
Withaferin A Tumor weight Influence on tumor growth 2.00 mg/kg HCT116 (xenograft) Mus musculus BALB/c
Withaferin A Tumor volume Influence on tumor growth 2.00 mg/kg HCT116 (xenograft) Mus musculus BALB/c
Withaferin A Body weight Influence on tumor growth 2.00 mg/kg HCT116 (xenograft) Mus musculus BALB/c